T. COMPLETE BOOK OF ROSES
Podwyszynska (1996) explained practical method for overcoming shoot senescence and difficulties with rooting of rose shoots propagated M vitro. Rooting was studied in micro cuttings of two miniature rose culti 'White Gem (difficult to root) and 'Starina' (Easy to root). Increasing the concentration of Co, Mg, Fe and MN in the medium markedly improved the quality of proliferated rose shoots.
Rout et. al., (1996) white working with the problem of tissue culture in rose demonstrated chlorpromazine induced in vitro bud break in Rosa Vivian cuItivar a ndora'. Shoot segment of rose cultivar landora' were cultured on M S supplemented with BA, Kinetin, GA, or the Calmodulin antagonist chlorpromazine (CPC) at various concentration. All the tested concentration of chlorpromazine (0.25 - 5.0 mg/liter) induced rapid bud break with 4-5 mg/liter being most effective. Shoot elongation showed a sirndar response to CPZ. The other was not more effective at pH 7.0 than at 4.0 and required calcium in the medium for optimal activity.
Syamal and Singh (1996) explained about in vitro propagation of rose in detail. Results of their experiment shows that shoot and nodal segments of R. hybricia cultivar 'Crimson Glory' proliferated when cultured on modified MS medium supplemented with various plant growth regulators. Nodal segments respond more rapidly and produced more shoots than shoot tips. Proliferated shoots produced the higher number of roots when transferred to half strength basal medium. Rooted plantlets were successfully transferred to pots under natural condition after acclimatization.
Salchi and Khosh-Khui (1997) studied tile effects of explant length and diameter 011 is vitro growth and proliferation rate in miniature rose cultivar 'Little Buckaroo' and 'Baby Masquerade' and 'Saurati'. Shoot length and proliferation rate were measured one and four weeks after culture. Best shoot development and proliferation rate was obtained from explant with the greatest length and diameter. In 'Little Buckaroo', a positive correlation was observed between shoot length after 1 week and proliferation rate after four week. Wardrops el. at, (1997) conducted an experiment and demonstrated that carbon dioxide gasseol fluorocarbon enhances micro propagation of rose (Rosa thinensis Incq). During the experiment the incent perflurochemical (PFC) liquid, perflurodecinin (touter PPG) was used to increase the CO, supply of the culture rose cultivar 'Baby Love" shoots. They have shown that the treatment of cultured shoot with CO, gasseol PFC also significantly reduced the accumulation of phenolic compound in roots. The total chlorophyll of aerial parts was unaffected although total protein in shoots and root was significantly lower than the control. The biotechnological implications of this novel cultural regime are discussed for the micro propagation of woody species.
Singh and Syamal (1999) conducted critical studies on the effect of growth regulator on in vitro shoot proliferation in Rosa x hybrida Lev. "Soma." for micro propagation. Singh and Syarnal (2000) determined that anti-auxins enhanve Rom hybrid:I L. micropropagation. Shoots of rose cultivars "Super Star" and "Sonia" were multiplied for 50 subcultures at 4-week intervals on solidified Murashige and Skoog medium supplemented with 22.19 plil ben.' aslrnimrov 1.07 pM NAA n 0.05 Of gibberellic acid.
No comments:
Post a Comment